We don't bother decolorizing H&Es since the IHC methodology generally takes out the histochemical staining....also, we're counterstaining with hematoxylin anyway. You should have no problem doing CK20 over an H&E.
Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Paula Lucas Sent: Monday, October 13, 2008 2:38 PM To: [EMAIL PROTECTED] Subject: [Histonet] Decolorize for IHC Hello, I need to decolorize an H&E stained section for Cytokeratin 20 and I would like to know the proper way of decolorizing for IHC. Is it even possible to get a CK 20 positive stain after doing so? Thanks, Paula Lucas HT Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
