I just did a CK20 on an H&E and Linda is correct, there is no need to decolorize before doing the CK20. The only thing I would suggest is that you are sure that all mounting media is removed after the coverslip comes off your H&E. The longer it's been coverslipped the more mindful of mounting media removal you should be.
Good Luck, Glen Dawson IHC Manager Milwaukee, WI -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] Behalf Of Sebree Linda A. Sent: Monday, October 13, 2008 2:47 PM To: Paula Lucas; [EMAIL PROTECTED] Subject: RE: [Histonet] Decolorize for IHC We don't bother decolorizing H&Es since the IHC methodology generally takes out the histochemical staining....also, we're counterstaining with hematoxylin anyway. You should have no problem doing CK20 over an H&E. Linda A. Sebree University of Wisconsin Hospital & Clinics IHC/ISH Laboratory DB1-223 VAH 600 Highland Ave. Madison, WI 53792 (608)265-6596 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Paula Lucas Sent: Monday, October 13, 2008 2:38 PM To: [EMAIL PROTECTED] Subject: [Histonet] Decolorize for IHC Hello, I need to decolorize an H&E stained section for Cytokeratin 20 and I would like to know the proper way of decolorizing for IHC. Is it even possible to get a CK 20 positive stain after doing so? Thanks, Paula Lucas HT Lab Manager Bio-Path Medical Group _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
