Helen Johnson asks: >>Does anyone have a protocol for staining rat vaginal smears with H&E?<<
Any H & E suitable for frozen sections should suffice. But it's the wrong stain, assuming you're looking for cornification (keratinization) of superficial squamous cells. I don't know what stain was used traditionally, but if I were to suggest something, it would be the ordinary Pap (Papanicolaou) stain, which stains nuclei with hematoxylin, intermediate cells with light green or fast green, and superficial cells with orange G. - If you buy a Pap stain commercially, get one in which the EA50 and the orange G are separate stains, and not combined. Bob Richmond Samurai Pathologist Knoxville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet