Hi Betsy:
Wow, a real histology posting! If it is any comfort I have had the
same problem with mouse heart after Zenker's fix (I wanted nice
intercalated disks). I think the solution is to use NBF.
Geoff
Molinari, Betsy wrote:
Hi,
I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in
70% for anywhere from 24-48hrs. They were processed on a short run. The
epicardium looks good but the mid myocardium is wavy.
15 min 80%
15 min 95 x 2
15 min 100% x 2
15 min Xylenes x2
10 min paraffin x 4
Any suggestions?
Thanks.
Betsy Molinari HT(ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
MC 1-283
Houston, TX 77030
832-355-6524
832-355-6812 (fax)
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583
[EMAIL PROTECTED]
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