Hi, I processed some mouse hearts that were in Z-Fix for 24 hrs. then put in 70% for anywhere from 24-48hrs. They were processed on a short run. The epicardium looks good but the mid myocardium is wavy.
15 min 80% 15 min 95 x 2 15 min 100% x 2 15 min Xylenes x2 10 min paraffin x 4 Any suggestions? Thanks. Betsy Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology 6770 Bertner Ave. MC 1-283 Houston, TX 77030 832-355-6524 832-355-6812 (fax) _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet