Dear Andrea, We stopped using the xylene substitute when my new co-worker started here. She doesn't like change and since she used only xylene at her last job, I compromised and switched back to using the real stuff. You have to choose your battles and since she would have set up additional stations to include the xylene anyway, I gave in.
I used to use the mounting medium from ThermoElectron, called Shandon Xylene Substitute Mountant. It was nicely miscible with the histoclear. I don't know the new catalog number since they have merged so many times with other companies. I guess you could search the Fisher Scientific website. Good luck, Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: [EMAIL PROTECTED] Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Andrea Grantham Sent: Tuesday, November 18, 2008 8:23 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] HistoClear question A student in my lab is doing some work in another lab here and having some problems with coverslipping their H&E sections. They are using HistoClear 1X as a clearing agent and Cytoseal mounting medium. From looking at the coverslipped slides I'm guessing that these two products are not compatible. The mounting medium is sort of clumping up under coverslips and has a hazy appearance. Yes, I did also find a bit of water in their HistoClear as well. Does anybody else have any experience with HistoClear 1X and/or does anybody know what mounting medium is good to use with the product? I suggested that they call the company who manufactures HistoClear but I don't know if they have done that yet. Thanks, Andi Grantham ..................................................................... : Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy : : Sr. Research Specialist University of Arizona : : (office: AHSC 4212) P.O. Box 245044 : : (voice: 520-626-4415) Tucson, AZ 85724-5044 USA : : (FAX: 520-626-2097) (email: [EMAIL PROTECTED]) : :...................................................................: http://www.cba.arizona.edu/histology-lab.html _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet