Andi:
Histoclear (in all its "variants" and names, like Histolene) is a d-Limonene
containing substance known to having incompatibility problems with mounting
media, specially those toluene containing and has to be used with the
proprietary mounting medium brand Histomount that is xylene based.
Besides that, Histoclear is notorious for fading hematoxylin, and is not as
good as xylene for dewaxing sections before IHC. Has a strong odor and has been
reported as hardening brain, liver and spleen. Has caused skin allergic
reactions and headaches.
René J.
--- On Tue, 11/18/08, Andrea Grantham <[EMAIL PROTECTED]> wrote:
From: Andrea Grantham <[EMAIL PROTECTED]>
Subject: [Histonet] HistoClear question
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, November 18, 2008, 11:23 AM
A student in my lab is doing some work in another lab here and having some
problems with coverslipping their H&E sections. They are using HistoClear 1X
as a clearing agent and Cytoseal mounting medium. From looking at the
coverslipped slides I'm guessing that these two products are not compatible.
The mounting medium is sort of clumping up under coverslips and has a hazy
appearance. Yes, I did also find a bit of water in their HistoClear as well.
Does anybody else have any experience with HistoClear 1X and/or does anybody
know what mounting medium is good to use with the product? I suggested that they
call the company who manufactures HistoClear but I don't know if they have
done that yet.
Thanks,
Andi Grantham
.....................................................................
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology & Anatomy :
: Sr. Research Specialist University of Arizona :
: (office: AHSC 4212) P.O. Box 245044 :
: (voice: 520-626-4415) Tucson, AZ 85724-5044 USA :
: (FAX: 520-626-2097) (email: [EMAIL PROTECTED]) :
:...................................................................:
http://www.cba.arizona.edu/histology-lab.html
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