Dear Histonetters, I'd like to ask for recommendations for anti-fluorophore antibodies. I'm planning to use a fluorophore-labelled primary antibody for mouse-on-mouse IHC. This is then detected using a labelled anti-rabbitIg (or whatever host it is) ab.
Apparently Dako has discontinued the unlabelled rabbit anti-FITC antibody that I understand used to be popular in these applications, and I was told the remaining HRP version doesn't work well for subsequent detection with an antiIg ab. Has anyone tried the rabbit anti-Alexa488 antibody by Molecular Probes / Invitrogen? Or are there any other good anti-FITC abs available? With best regards, Mikael Niku ----------------------------------------------------- Mikael Niku, PhD, university lecturer University of Helsinki, Division of Nutrition URL: mikael.nikunnakki.info - What do I think of western civilization? I think it would be a good idea! Gandhi ----------------------------------------------------- _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet