Mouse tissues, any one, will dry out too much with ethanol and xylene. I advise you to stop using ethanol and xylene and substitute BOTH with iso-propanol at the concentrations and times you are using now. Instead of the first paraffin, use a mixture 1:1 of iso-propanol and paraffin, followed by the 2 paraffins. Try it and you will notice the difference (and the saings). René J.
--- On Wed, 12/10/08, Kathleen Roberts <[EMAIL PROTECTED]> wrote: From: Kathleen Roberts <[EMAIL PROTECTED]> Subject: [Histonet] Processing mouse seminal vesicles To: "'histonet@lists.utsouthwestern.edu'" <histonet@lists.utsouthwestern.edu> Date: Wednesday, December 10, 2008, 11:54 AM Does anybody have a protocol for this? My last batch of these came out VERY dry and crunchy when run with other tissues on my standard protocol, which is as follows: (They are fixed on the benchtop in 10% NBF for 4-5 days, then rinsed out before processing.) 70%: 30 min 80%: 30 min 95%: 45 min 95%: 45 min 100%: 45 min 100%: 45 min xylene: 45 min xylene: 45 min Paraffin: 30 min Paraffin: 30min Paraffin: 30 min My other thought is that something is up with our VIP 5 processor, though no error messages are showing up. Any and all suggestions are most welcome. Thanks in advance, Kathleen Roberts Rutgers University _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
