I personally prefer to wash out longer - until the flow out turned to be clear.
Or you will have some blood cells inside the liver/brain, depending on your tissues of interest. Or for IHC staining this might arise some problems. 2008-12-11 tf 发件人： Geoff McAuliffe 发送时间： 2008-12-11 05:20:24 收件人： Neil Fournier 抄送： histonet@lists.utsouthwestern.edu 主题： Re: [Histonet] perfusion question Does not matter. The only error I see people making is spending a lot of time on the washout step. You will never get all of the blood out so there is no reason to try. Given a 250 g rat, I would use 25-30 ml of washout instilled in 15-20 seconds, then switch over to fix. Geoff Neil Fournier wrote: > Is there a rationale for using normal saline (0.9% (w/v) NaCl dissolved in > dH2O) over 0.1 M PBS (pH 7.4) as a rinsing solution during intraventricular > perfusion of a rat. Would one yield better results over the other? > > Also is there a raionale for why some people perfuse using PBS made only from > monobasic and dibasic sodium phosphate (with 0.9% NaCl) vs. using PBS that > also include KCl, sodium phosphate dibasic, NaCl, and potassium phosphate > monobasic in the recipe. > > Thanks for the help > > Neil > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732)-235-4583 [EMAIL PROTECTED] ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu
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