I use Dako's PCNA (cat. # M0789), mouse monoclonal, clone PC10, HIER with
pressure cooker (Decloaker, 30" at 121C, 21psi) in Target Retrieval buffer,
45 min in primary antibody (current dilution is 1:200, but that varies with
each new lot), 30 min in goat anti-Ms EnVision+/HRP, 15 min in AEC. I use
an autostainer, but it can be done manually with the same incubation times
at RT.
This works for me on many mammalian species.
Jan Shivers
UMN VetDiagLab
St. Paul, MN
----- Original Message -----
From: "Merced Leiker" <[EMAIL PROTECTED]>
To: <[email protected]>
Sent: Wednesday, December 10, 2008 1:07 PM
Subject: [Histonet] PCNA staining on paraffin
Does anyone have any suggestions for staining PCNA on paraffin? We are
using Santa Cruz's PCNA (FL261) SC-7907.
We have already stained with it successfully on frozen sections, but it
does not appear to be working on paraffin. Thank you.
Merced M Leiker
Research Technician II
354 BRB (pkgs) / 140 Farber Hall (letters)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725
"Without my flaws I'm really very boring."
- random internet blog commentator
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
[email protected]
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
