Second this, the DAKO clone works great. -----Original Message----- From: Jan Shivers <[EMAIL PROTECTED]>
Date: Thu, 11 Dec 2008 10:01:38 To: Merced Leiker<[EMAIL PROTECTED]> Cc: histonet<histonet@lists.utsouthwestern.edu> Subject: Re: [Histonet] PCNA staining on paraffin I use Dako's PCNA (cat. # M0789), mouse monoclonal, clone PC10, HIER with pressure cooker (Decloaker, 30" at 121C, 21psi) in Target Retrieval buffer, 45 min in primary antibody (current dilution is 1:200, but that varies with each new lot), 30 min in goat anti-Ms EnVision+/HRP, 15 min in AEC. I use an autostainer, but it can be done manually with the same incubation times at RT. This works for me on many mammalian species. Jan Shivers UMN VetDiagLab St. Paul, MN ----- Original Message ----- From: "Merced Leiker" <[EMAIL PROTECTED]> To: <histonet@lists.utsouthwestern.edu> Sent: Wednesday, December 10, 2008 1:07 PM Subject: [Histonet] PCNA staining on paraffin > Does anyone have any suggestions for staining PCNA on paraffin? We are > using Santa Cruz's PCNA (FL261) SC-7907. > > We have already stained with it successfully on frozen sections, but it > does not appear to be working on paraffin. Thank you. > > Merced M Leiker > Research Technician II > 354 BRB (pkgs) / 140 Farber Hall (letters) > School of Medicine and Biomedical Sciences > State University of New York at Buffalo > 3435 Main St, Buffalo, NY 14214 > Ph: (716) 829-6033 > Fx: (716) 829-2725 > > "Without my flaws I'm really very boring." > - random internet blog commentator > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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