Use a PC10 mouse clone for pwax sections: works very well on many species that have been fixed in routine Formalin. Imho, no special procedures reqd. Sure, AR is best. If you wish to check out this site: http://www.immunoportal.com/index.php you might find further help? Also , be very careful if you use PCNA as a proliferation marker......it has a very long half-life compared with Ki67. So, use an anti Ki67 Ab instead for general proliferation studies that cannot include use of BrdU. Sure, if you want to assess a Mitotic index...use pH3 Ab on pwax sections. carl
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