Dear Histo-Experts, I'm interested in paraffin processing protocols for rat kidneys, spleens and livers. Specifically, I'm interested in fixation time (immersion fixation in formalin) and dehydration and paraffin infiltration times. I've done some using my best guess based on the thickness of the tissue and resulting sections (5-7um) have "spaces" in them (after H and E) which may be due to inadequate fixation or dehydration times (I'm guessing). The spaces don't look like tears from sectioning...they look more like tissue separation... If you wouldn't mind sharing your protocols with me that would be WONDERFUL!!! A thousand thanks in advance! danielle
Danielle Crippen Morphology and Imaging Core _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet