I have been handling Bouin fixed animal tissue for years. We removed the Bouins picric acid yellow by submerging our specimens in 70% Alcohol saturated with Lithium Carbonate. It usually takes overnight. I have had PI's bring me the samples after rinsing their samples in Lithium Carbonate solution overnight, this even worked better, after rinsing with the Lithium Carbonate saturated Aqueous Solution they placed them in 70% and brought them to me for processing. Gail 's processing is right on. Have you seen the Animal Tissue Book that Gail and Diane Sterchi published? It is very helpful. I believe you can obtain it through NSH
Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Monday, February 02, 2009 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bouin's rinsing protocol Hello. I am looking for information regarding procedures for tissue handling after Bouin's fixation, prior to processing in a standard automated tissue processor (VIP). We are handling pig, mouse, rat, and human samples, and will fix in Bouin's for 48-72 hrs. I've been told to rinse until all yellow is washed out, and people generally rinse in 70% alcohol or water. Sometimes it take days to wash all of the picric acid out. Thank you so much for your wealth of information! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet