What mounting media are you using? Are the slides left to dry near a UV light from a hood or cryostat?
-----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of histonet-requ...@lists.utsouthwestern.edu Sent: Tuesday, February 03, 2009 12:52 PM To: histonet@lists.utsouthwestern.edu Subject: Histonet Digest, Vol 63, Issue 4 Send Histonet mailing list submissions to histonet@lists.utsouthwestern.edu To subscribe or unsubscribe via the World Wide Web, visit http://lists.utsouthwestern.edu/mailman/listinfo/histonet or, via email, send a message with subject or body 'help' to histonet-requ...@lists.utsouthwestern.edu You can reach the person managing the list at histonet-ow...@lists.utsouthwestern.edu When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..." Today's Topics: 1. Re: IHC and ISH (Aprill Watanabe) 2. RE: NY State Inspection Checklist (Norm Burnham) 3. query regarding IHC slide (shazana hilda) 4. Re: perplexed (Piero Nelva) 5. RE: Bouin's rinsing protocol (Swain, Frances L) 6. RE: query regarding IHC slide (Smith, Allen) 7. RE: perplexed (Terri Braud) 8. Re: query regarding IHC slide (Jan Shivers) 9. RE: Who can gross?? (Ingles Claire ) 10. RE: cryostat adapter (YourBiomed.Com ) 11. RE: IHC and ISH (YourBiomed.Com ) 12. cryostat adapter. (mari.ann.mailh...@leica-microsystems.com) 13. Re: IHC and ISH (Tora Bardal) 14. RE: Histo gel Issues (Jo Dee Fish) 15. Hoescht and Giemsa (Xenophanes _) 16. DAPI with DAB (Reza Farivar-Mohseni, Dr) 17. Self-Charging Microscope Slides (Kelvin Poon) ---------------------------------------------------------------------- Message: 1 Date: Mon, 02 Feb 2009 16:19:23 -0700 From: Aprill Watanabe <awatan...@tgen.org> Subject: [Histonet] Re: IHC and ISH To: <histonet@lists.utsouthwestern.edu> Message-ID: <c5accd8b.968b%awatan...@tgen.org> Content-Type: text/plain; charset="US-ASCII" Bond from Leica. It's a great machine and very user friendly. The training was great and effective. The technical staff helps in so many ways. I've had my machine for 3 years and would get another one if we increased our volume. On 2/2/09 4:16 PM, "histonet-requ...@lists.utsouthwestern.edu" <histonet-requ...@lists.utsouthwestern.edu> wrote: Aprill Watanabe, B.S. Research Associate Integrated Cancer Genomics Division Tissue Microarray Center (TMA) Translational Genomics Research Institute (TGen) main: 602-343-8822 Fax: 602-343-8840 awatan...@tgen.org www.tgen.org ------------------------------ Message: 2 Date: Mon, 2 Feb 2009 17:21:50 -0600 From: "Norm Burnham" <norm.burn...@propath.com> Subject: RE: [Histonet] NY State Inspection Checklist To: <thisis...@aol.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <82c7248978cb50469fd6ba68ebbefe67f4c...@exchange.propathlab.com> Content-Type: text/plain; charset="us-ascii" http://www.wadsworth.org/labcert/clep/standards.htm Norm Burnham ProPath www.ProPath.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of thisis...@aol.com Sent: Monday, February 02, 2009 5:12 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] NY State Inspection Checklist I was just informed that NY now has an inspection checklist.? Can someone let me know where I can find it. Thanks, Ann _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 3 Date: Mon, 2 Feb 2009 18:10:33 -0800 (PST) From: shazana hilda <hilda_1...@yahoo.com> Subject: [Histonet] query regarding IHC slide To: histonet@lists.utsouthwestern.edu Message-ID: <653484.44401...@web38805.mail.mud.yahoo.com> Content-Type: text/plain; charset=us-ascii Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it? Any response are greatly appreciated. Regards. Shazana Hilda ShamsuddinUniversiti Sains Malaysia, Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1...@yahoo.com ------------------------------ Message: 4 Date: Tue, 3 Feb 2009 19:35:20 +1100 From: "Piero Nelva" <pieronelv...@bigpond.com> Subject: Re: [Histonet] perplexed To: <histonet@lists.utsouthwestern.edu> Message-ID: <ef2daabfcf504d089535e9f424196...@pentium4> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Hi Angela There was a problem with the heating pads for a certain batch of Ventana XT's. We had uneven heating for a week or so until the rep replaced the entire pad. You can check if your machine is one that was made in the offending batch. No problems since. Regards Piero Nelva Anatomical Pathology Monash Medical Centre Victoria Australia ----- Original Message ----- From: "Angela Bitting" <akbitt...@geisinger.edu> To: <histonet@lists.utsouthwestern.edu> Sent: Tuesday, February 03, 2009 7:25 AM Subject: [Histonet] perplexed Here's one for the Ventana BenchmarkXT users out there: We mount the patient tissue on the same slide with our control tissue. The control stains beautifully, but there is absolutely no staining in the patient tissue. This will happen to one or two out of 30 slides on a run. It also happened on two different instruments. Over the last week this has occurred 6 times. Different protocols, different machines. I tend to think its a deparaffinization issue. Can one side of a pad heat and the other end not reach temp? We haven't noticed that the slides moved during staining or were not seated properly on the pad. Help me figure this one out folks!! Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------------------------------------------------------------------------- > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -------------------------------------------------------------------------------- No virus found in this incoming message. Checked by AVG - www.avg.com Version: 8.0.233 / Virus Database: 270.10.16/1930 - Release Date: 01/31/09 20:03:00 ------------------------------ Message: 5 Date: Tue, 3 Feb 2009 06:39:03 -0600 From: "Swain, Frances L" <swainfranc...@uams.edu> Subject: [Histonet] RE: Bouin's rinsing protocol To: "Jennifer Anderson" <jander...@halozyme.com>, "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <5b6165d78ac14544974a844787b47e380173973...@mail5.ad.uams.edu> Content-Type: text/plain; charset=us-ascii I have been handling Bouin fixed animal tissue for years. We removed the Bouins picric acid yellow by submerging our specimens in 70% Alcohol saturated with Lithium Carbonate. It usually takes overnight. I have had PI's bring me the samples after rinsing their samples in Lithium Carbonate solution overnight, this even worked better, after rinsing with the Lithium Carbonate saturated Aqueous Solution they placed them in 70% and brought them to me for processing. Gail 's processing is right on. Have you seen the Animal Tissue Book that Gail and Diane Sterchi published? It is very helpful. I believe you can obtain it through NSH Frances L. Swain HT(ASCP) A. A. S. Special Procedures Technician Department of Orthopaedic Surgery Center for Orthopaedic Research Barton Research Building 2R28 4301 West Markham Street Little Rock AR 72205 (501) 686-8739 PHONE (501) 686-8987 FAX swainfranc...@uams.edu email -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jennifer Anderson Sent: Monday, February 02, 2009 3:10 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Bouin's rinsing protocol Hello. I am looking for information regarding procedures for tissue handling after Bouin's fixation, prior to processing in a standard automated tissue processor (VIP). We are handling pig, mouse, rat, and human samples, and will fix in Bouin's for 48-72 hrs. I've been told to rinse until all yellow is washed out, and people generally rinse in 70% alcohol or water. Sometimes it take days to wash all of the picric acid out. Thank you so much for your wealth of information! Jennifer M. Anderson, Scientist Halozyme Therapeutics, Inc. 11404 Sorrento Valley Road San Diego, CA 92121 858-704-8333 jander...@halozyme.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message. ------------------------------ Message: 6 Date: Tue, 3 Feb 2009 08:51:26 -0500 From: "Smith, Allen" <asm...@mail.barry.edu> Subject: RE: [Histonet] query regarding IHC slide To: 'shazana hilda' <hilda_1...@yahoo.com> Cc: "'Histonet@lists.utsouthwestern.edu'" <Histonet@lists.utsouthwestern.edu> Message-ID: <e4132130ac2f764d8c173c5400d5304290e7846...@exchsrv02.barrynet.barry.edu> Content-Type: text/plain; charset="us-ascii" A lot depends on the stain chosen. I have used Vector's Nova Red for the last 3 years. I have seen no fading of my older Nova Red slides. Prof. Allen A. Smith Barry University School of Podiatric Medicine Miami Shores, Florida -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of shazana hilda Sent: Monday, February 02, 2009 9:11 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] query regarding IHC slide Dear All, Is anyone has experience with faded IHC-slide stained? I've done my IHC stained on few markers and after 3-4 months I viewed the slide and it seems that the stain became faded.Does anyone has opinion regarding this matter and how to troubleshoot it? Any response are greatly appreciated. Regards. Shazana Hilda ShamsuddinUniversiti Sains Malaysia, Off. no: +609- 766 4440 Fax no: +609- 765 3370 Email: hilda_1...@yahoo.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 7 Date: Tue, 3 Feb 2009 09:58:19 -0500 From: "Terri Braud" <tbr...@holyredeemer.com> Subject: [Histonet] RE: perplexed To: <histonet@lists.utsouthwestern.edu> Message-ID: <f3d3b1ce184aa34abb007c3e0fdcc38403848...@hrex-svr.holyredeemer.local> Content-Type: text/plain; charset="iso-8859-1" 10. perplexed (Angela Bitting) histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and ISH Hi everyone, I am currently in the process of evaluating IHC equipment to replace my old Dako Autostainers. The pathologists I work for are interested in bringing ISH into our lab. My questions are, what IHC instrument have you found works the best for IHC and ISH, and what kind of training did your personnel go through to become qualified to perform ISH? Thank you! Jean Taylor, HT(ASCP)QIHC ICH Tech Meriter Labs Madison, WI We had the same issue here with our slides, even though the control tissue was mounted on the same slide, we would get decent control staining but no patient, or vice versa. I spoke with several Ventana tech folk, and the one thing that they insisted on, was the brand of slide I was using. We never used the "control" slides with the little red box, just charged slides from another company than what Ventana was recommending. As a tech with over 30 years of experience, I couldn't imagine that one plus charged slide could be so different from the other and that it would contribute that much difference to how tissue would stain. After all, the tissue was still stuck fast, and no one could explain the principle behind what they were saying. But hey - I was wrong, wrong, wrong. Once we switched to Cardinal's Superfrost Plus slides instead of another company's "charged" slides, we never had the problem again. It still doesn't make sense, and no one can explain why, but it did seem to make a difference. I hope this helps you. Terri Terri L. Braud, HT(ASCP) Anatomic Pathology Supervisor Laboratory Holy Redeemer Hospital and Medical Center 1648 Huntingdon Pike Meadowbrook, PA 19046 (215) 938-3676 phone (215) 938-3689 fax --------------------------------------------------------------------------------- CONFIDENTIALITY NOTICE: This E-Mail is intended only for the use of the individual or entity to which it was sent. It may contain information that is privileged and/or confidential, and the use or disclosure of such information may also be restricted under applicable federal and state law. If you received this communication in error, please do not distribute any part of it or retain any copies, and delete the original E-Mail. Please notify the sender of any error by E-Mail. Thank you for your cooperation. ------------------------------ Message: 8 Date: Tue, 3 Feb 2009 09:12:09 -0600 From: "Jan Shivers" <shive...@umn.edu> Subject: Re: [Histonet] query regarding IHC slide To: "shazana hilda" <hilda_1...@yahoo.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <69fa5aad53d2495cad2ff3010a6ce...@auxs.umn.edu> Content-Type: text/plain; format=flowed; charset="iso-8859-1"; reply-type=original Please tell us what chromogen stains and counterstains you are using, and the type of mounting medium for the coverslip. This will help us try to answer your question. Jan Shivers UMN VDL ----- Original Message ----- From: "shazana hilda" <hilda_1...@yahoo.com> To: <histonet@lists.utsouthwestern.edu> Sent: Monday, February 02, 2009 8:10 PM Subject: [Histonet] query regarding IHC slide > Dear All, > Is anyone has experience with faded IHC-slide stained? I've done my IHC > stained on few markers and after 3-4 months I viewed the slide and it > seems that the stain became faded.Does anyone has opinion regarding this > matter and how to troubleshoot it? > > Any response are greatly appreciated. > > > Regards. > > Shazana Hilda ShamsuddinUniversiti Sains Malaysia, > Off. no: +609- 766 4440 > Fax no: +609- 765 3370 > Email: hilda_1...@yahoo.com > > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 9 Date: Tue, 3 Feb 2009 09:39:29 -0600 From: "Ingles Claire " <cing...@uwhealth.org> Subject: RE: [Histonet] Who can gross?? To: "Judith L. Williams" <jud...@u.washington.edu>, "Charles.Embrey" <charles.emb...@carle.com> Cc: histonet <histo...@pathology.swmed.edu> Message-ID: <f2f030053f9b7345831bed293a6d57e109a...@uwhc-mail01.uwhis.hosp.wisc.edu> Content-Type: text/plain; charset="iso-8859-1" That's why we always keep at least one tall person on staff to reach the top shelves, etc. :) Claire ________________________________ From: histonet-boun...@lists.utsouthwestern.edu on behalf of Judith L. Williams Sent: Fri 1/30/2009 11:59 AM To: Charles.Embrey Cc: histonet Subject: RE: [Histonet] Who can gross?? Now that folks is a beautiful Friday statement and comeback - those darn short techs really cause problems don't they :/ hehehehe Judy On Fri, 30 Jan 2009, Charles.Embrey wrote: > As short histo techs what kind of aids are you using, stepladders or > stools? Sorry, I couldn't resist. There is a ton of information on the > histonet archive about this. A search should yield all the info you > seek and more. > > ------------------------------ Message: 10 Date: Tue, 3 Feb 2009 7:51:30 -0800 From: "YourBiomed.Com " <yourbio...@cox.net> Subject: RE: [Histonet] cryostat adapter To: histonet@lists.utsouthwestern.edu Message-ID: <20090203105130.eh2tu.1015850.im...@fed1rmwml37> Content-Type: text/plain; charset=utf-8 We have the adapter here at IMEB, Inc. Please go to www.imebinc.com for our contact info and ask for Brad. Thank you ------------------------------ Message: 11 Date: Tue, 3 Feb 2009 7:52:59 -0800 From: "YourBiomed.Com " <yourbio...@cox.net> Subject: RE: [Histonet] IHC and ISH To: histonet@lists.utsouthwestern.edu Message-ID: <20090203105259.j7m4v.1015899.im...@fed1rmwml37> Content-Type: text/plain; charset=utf-8 Jean, I work at IMEB, Inc in San Marcos, CA. I'm very familiar with the instruments listed in the responses to your question. What it comes down to is cost, closed system or open system (use your reagents or theirs (Vendors). The Leica BondMax has it strengths and weaknesses as does the Ventana and Intellipath from BioCare. They all offer great training and I'm sure you'll get the end result (staining) to meet your needs. But do you want to buy the majority of your reagents from the vendor selling the instrument; which can be costly...depending on your quantity of slides your lab processes or open source in which you can mix and match the reagents that you prefer. This can or in some cases may not be a cost saver. Any questions, please contact me. Brad IMEB, Inc www.imebinc.com -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean Sent: Monday, February 02, 2009 9:51 AM To: ih...@googlegroups.com; ih...@yahoogroups.com; histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC and ISH Hi everyone, I am currently in the process of evaluating IHC equipment to replace my old Dako Autostainers. The pathologists I work for are interested in bringing ISH into our lab. My questions are, what IHC instrument have you found works the best for IHC and ISH, and what kind of training did your personnel go through to become qualified to perform ISH? Thank you! Jean Taylor, HT(ASCP)QIHC ICH Tech Meriter Labs Madison, WI _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 12 Date: Tue, 3 Feb 2009 10:06:45 -0600 From: mari.ann.mailh...@leica-microsystems.com Subject: [Histonet] cryostat adapter. To: lc...@mednet.ucla.edu Cc: histonet@lists.utsouthwestern.edu Message-ID: <of486fae44.7512d3e8-on86257552.005448a4-86257552.00589...@leica-microsystems.com> Content-Type: text/plain; charset=US-ASCII Leslie I contacted our collegues in Germany and was given the below nformation on the inner demensions of the Miles adapter for the CM1850 cryostat by Leica for use with embedding rings. The inner dimensions of the adapter are 2,3 cm x 2,3 cm. If you have other questions please feel free contact. Kind Regards Mari Ann Mailhiot BA HT ASCP Application Specialist/Trainer Leica Microsystems Biosystems Division Technical Assistance Center 800 248 0123 x7267 847 236 3063 fax mari.ann.mailh...@leica-microsystems.com www.leica-microsystems.com ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ ------------------------------ Message: 13 Date: Tue, 03 Feb 2009 17:40:46 +0100 From: Tora Bardal <tora.bar...@bio.ntnu.no> Subject: Re: [Histonet] IHC and ISH To: "YourBiomed.Com" <yourbio...@cox.net> Cc: histonet@lists.utsouthwestern.edu Message-ID: <4988738e.5030...@bio.ntnu.no> Content-Type: text/plain; charset=UTF-8; format=flowed Does anyone have experience with InsituPro? http://www.intavis.com/en/In_Situ_Detection/index.php Tora YourBiomed.Com wrote: > Jean, > > I work at IMEB, Inc in San Marcos, CA. > I'm very familiar with the instruments listed in the responses to your > question. > What it comes down to is cost, closed system or open system (use your > reagents or theirs (Vendors). > The Leica BondMax has it strengths and weaknesses as does the Ventana and > Intellipath from BioCare. > They all offer great training and I'm sure you'll get the end result > (staining) to meet your needs. > But do you want to buy the majority of your reagents from the vendor selling > the instrument; which can be costly...depending on your quantity of slides > your lab processes or open source in which you can mix and match the reagents > that you prefer. This can or in some cases may not be a cost saver. > > Any questions, please contact me. > > Brad > IMEB, Inc > www.imebinc.com > > > -----Original Message----- > From: histonet-boun...@lists.utsouthwestern.edu > [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Taylor, Jean > Sent: Monday, February 02, 2009 9:51 AM > To: ih...@googlegroups.com; ih...@yahoogroups.com; > histonet@lists.utsouthwestern.edu > Subject: [Histonet] IHC and ISH > > Hi everyone, > > I am currently in the process of evaluating IHC equipment to replace my old > Dako Autostainers. The pathologists I work for are interested in bringing ISH > into our lab. My questions are, what IHC instrument have you found works the > best for IHC and ISH, and what kind of training did your personnel go through > to become qualified to perform ISH? > > Thank you! > > Jean Taylor, HT(ASCP)QIHC > ICH Tech > Meriter Labs > Madison, WI > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > ------------------------------ Message: 14 Date: Tue, 3 Feb 2009 08:49:41 -0800 From: "Jo Dee Fish" <jf...@gladstone.ucsf.edu> Subject: RE: [Histonet] Histo gel Issues To: "'pam plumlee'" <paw...@yahoo.com>, <histonet@lists.utsouthwestern.edu> Message-ID: <1df34928912d42a39b266eeb5a28c...@jfish> Content-Type: text/plain; charset="us-ascii" Dear Pam, I've had the same problem. I called Richard-Allen just after they were "absorbed" by Thermo Fisher and got no answers. They had never heard of such a problem and didn't know how to solve it. I have heard from another user that had the same exact problem. I lost precious samples, E6.5 mouse embryos, because of this "drying out and hardening" of the histogel. I had to stop using it all together. If anyone has any suggestions, please let us know! Take care Pam and all histonetters, Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jf...@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of pam plumlee Sent: Monday, February 02, 2009 1:06 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Histo gel Issues Dear Group: I'm having problems with the processing of small tissues in histogel. I follow the suggested embedding directions on the package and then process the gel blocks. The results are very inconsistent-ideally, I'll get nice soft gel blocks-but, usually in a batch of 10 I get 2 good blocks and 8 dried up, flat hard squares. They are all handled and processed the same. Anyone experience this before? Thanks for any input. Pam Plumlee H.T. Pfizer La Jolla pam.plum...@pfizer.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 3 Feb 2009 16:52:23 +0000 From: Xenophanes _ <xenophane...@hotmail.com> Subject: [Histonet] Hoescht and Giemsa To: <histonet@lists.utsouthwestern.edu> Message-ID: <bay130-w334d988bafbee1aa2a528cd2...@phx.gbl> Content-Type: text/plain; charset="Windows-1252" Hello all. I am excited to be a new participant of the Histonet list serve. I hope that you can provide some assistance in a little problem I am having.Simply, I wish to photograph blood smear slides (methanol fixed) with both Hoescht and Giemsa. I want to find items via normal light microscopy from the Giemsa stain and visualize the Hoescht via our UV imager on the same scope. The problem I am having is that performing the Giemsa stain first doesn't work out well because the Hoescht staining solution leeches out the Giemsa and produces washed out samples with little purple staining. I am trying to do the Hoescht stain first, but I am afraid that the Giemsa staining conditions might reduce the ability to resolve the fluorescent signal from the Hoescht dye.Furthermore, Hoescht binds DNA in the minor groove, while the Giemsa binds DNA on the phosphate groups. Does any one know about possible inhibition of binding between these two compounds since they are physically clos! e on the DNA strand itself?Thank you again for your time and assistance!-Blood Smear-O-Rama _________________________________________________________________ Windows Live?: E-mail. Chat. Share. Get more ways to connect. http://windowslive.com/explore?ocid=TXT_TAGLM_WL_t2_allup_explore_012009 ------------------------------ Message: 16 Date: Tue, 3 Feb 2009 12:06:10 -0500 From: "Reza Farivar-Mohseni, Dr" <reza.fari...@mail.mcgill.ca> Subject: [Histonet] DAPI with DAB To: "histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Message-ID: <8ea9e02db46b6e498a9d47891ba9e98d102...@exmbxvs4b.campus.mcgill.ca> Content-Type: text/plain; charset="iso-8859-1" Hello, I'm thinking of counterstaining some DAB-stained sections with DAPI. Does anyone know if DAPI will still bind with DAB stained cells and fluoresce with permount? Thanks, Reza ------------------------------ Message: 17 Date: Tue, 03 Feb 2009 17:42:00 +0000 From: Kelvin Poon <kelvin.p...@dit.ie> Subject: [Histonet] Self-Charging Microscope Slides To: histonet@lists.utsouthwestern.edu Message-ID: <498881e8.1080...@dit.ie> Content-Type: text/plain; charset=ISO-8859-1; format=flowed Hi all, I was wondering if anyone was familiar with the protocol used by manufacturers such as Menzel-Glaser regarding placing a permanent positive charge on their glass slides. In the case of M-G they are called Superfrost PLUS or ULTRA PLUS slides. The reason I am asking is because I wish to positively charge /quartz/ slides, rather than glass ones. Quartz is needed for my spectroscopy experiments. I am aware that it is relatively easy to self-coat slides if you wish to use poly-l-lysine or silane but both will show up as background interference in my case. I am actually unaware if and how well quartz can actually hold a charge. Any help appreciated! Thanks, Kelvin -- _______________________________________________* *Dr. Kelvin W. C. Poon Postdoctoral Research Fellow *Radiation and Environmental Science Centre* *focas** institute*** dublin institute of technology camden row, dublin 8 Ireland This message has been scanned for content and viruses by the DIT Information Services E-Mail Scanning Service, and is believed to be clean. http://www.dit.ie ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 63, Issue 4 *************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet