Hi Histonians, I have experienced some difficulties in doing snapped freeze muscle biopsies that need to be stained with the Modified Gomori's Trichrome by Engel, W.K., and Cunningham 1963. When I stained the frozen tissue that has not been post or pre fixed, I am getting red areas within this bundles that also have green in it. I know it should be solid green with reddish nuclei and red intermyofibrillar muscle. I truly believe this is an artifact. I checked the histonet archives and there has been much talk about the stain but not how to correct this artifact Any help will be appreciated.
John _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
