Hi Histo People, I tried to H&E with a Vector Lab's Gill's Formulation of Hematoxylin. Everything E'd just fine but there is no H.
Is there a way to go back and re-H after coverslipping? As a clarifier I used 1% glacial acetic acid for 20 seconds and blueing was saturated Lithium carbonate. I am using Gill's of unknown age (for 1 minute), the slides seemed sort of light after the water rinses but I went on thinking it would blue later. Nope-there is no nuclear stain at all. Can I go back and fix this? Remove coverslips, rehydrate and re-Hematoxylin? Please give me any suggestions you can think of. These blocks were a pain in the rear to section and I'd hate to have to resection them. Boo-Hoo & Waaah, Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core Microscope Facility, room B141 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet