Ammunition...no, was more just inquiring what the what the average would be and if there was any specific reason for thinner sections other that just preference.
Thanks Vanessa On 3/3/09 9:37 AM, "Rene J Buesa" <[email protected]> wrote: > Vanessa: > Lymph nodes for cellular details (special request) = 3 µm > H&E and all other special procedures = 5 µm > Sections for bone marrow and liver reticulum stain = 7 µm > Brain and central nervous system = 10 µm > > Now a question, why do you want to know this? To have "ammunition" to > challenge what is done in your new lab? Not a wise move. I don't think that > they would mind if you cut thinner, but they will mind if you start bringing > this issue about. Let your thinner sections "speak for themselves". It will > get the moment that "by example" your way will prevail. > René J. > > --- On Tue, 3/3/09, Vanessa J. Phelan <[email protected]> wrote: >> From: Vanessa J. Phelan <[email protected]> >> Subject: [Histonet] Section thickness >> To: [email protected] >> Date: Tuesday, March 3, 2009, 9:14 AM >> >> Hi Guys, >> >> Just wondering what thickness you cut sections at? I was always used to >> cutting at 2-3 microns in my last lab, however in my new place they are >> cutting at 6 microns (for both H & Es and IHC), which seems to me as really >> quite thick! What would be the average cutting thickness? >> >> Thanks a mill, >> >> Vanessa >> >> >> _______________________________________________ >> Histonet mailing list >> [email protected] >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
