Hi Colleen,
Although I agree that you have to make sure your
markers are good for the particular species you
work with, I respectfully disagree that CD34 does
not "work" in mouse.
CD34 is one of the best vascular markers for
mouse paraffin sections, of course it is
absolutely tissue specific. In mouse brain,
specifically, it is the best pan-EC marker I have
encountered.
Thanks,
Andrea
At 10:58 AM -0600 3/5/09, Colleen Forster wrote:
Again, you need to be sure that it will work in
mouse samples. CD34 is another marker that does
not work well in mouse.
Colleen Forster
anh2...@med.cornell.edu wrote:
You could use CD31 but would need to digest
first as it doesn't work well in fixed tissue
without enzymatic retrieval.
CD34 is a gem for brain vessels in paraffin so
might work in fixed frozen as well.
Andrea
------Original Message------
From: iskaliora
Sender: histonet-boun...@lists.utsouthwestern.edu
To: histonet@lists.utsouthwestern.edu
Cc: ɣɩɣɴÉ* É®ÉÉ´ÉÉÉ©ÉüÉ®É*
Sent: Mar 5, 2009 5:17 AM
Subject: [Histonet] staining brain vessels
I was wondering if anybody might have an idea
with the following problem we are
experiencing: we want to stain for blood
vessels in sections of mouse brain. Our
experimental tissues have been fixed overnight
in 4% paraformaldehyde and have been sitting in
PBS since.
We have tried staining with antibodies against
desmin, SMA, and collagen but we get NO
specific signal. We recently tried a non-fixed
mouse brain and got desmin to work immediately.
The problem is that we need to use the fixed
brains because they are our experimental model
and it would take too long (2 years to be
exact) to generate the same samples. If
anybody has come across such a problem before,
or has a specific protocol for vessels that
works on PFA fixed brain, we would appreciate
the suggestions!
thanks in advance!
Irini
-----------------------------------------------------
Irini Skaliora, PhD
Investigator C? (Assistant Professor)
Developmental Biology Division
Biomedical Research Foundation of the Academy of Athens (BRFAA)
Soranou Efessiou 4
Athens 11527
tel. +30-210-6597203 (office)
tel. +30-210-6597482 (lab)
fax. +30-210-6597545
email: iskali...@bioacademy.gr
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