Aldehyde fuchsin! Much simpler than Mallory Azan and very specific. You will need paraldehyde, a controlled substance, to make this stain. Alternatively, you can use acetaldehyde but you will need to triple the amount to get the molar equivalents correct. See "Staining properties of Aldehyde fuchsin analogues" by Buehner, Nettleton and Longley, J. Histochem. Cytochem.
27(3):782-787, 1979.

Geoff

Michele Wich wrote:
Can I please get some suggestions for histological stains that
demonstrate pancreatic beta cells? I need something that would be highly
specific without using an IHC method. Any advice is greatly appreciated!

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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 mcaul...@umdnj.edu
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