Unna's modified van Gieson's stain for the Rapid Bone Stain is reported to give deeper colors than the VG recipe/solution commonly found in histotechnology textbooks. It contains a higher concentration of dyes but also contains nitric acid which is going to acid etch the surface of the bone a bit. The resulting counterstain will have deeper colors. Be aware that commercial solutions of van Giesons might give weaker staining.
Gayle Callis HTL(ASCP)HT,MT Bozeman MT 59715 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cathy Mayton Sent: Thursday, March 19, 2009 2:33 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Sanderson's Rapid Bone Stain Dear Fellow Histonetters, There are 3 counterstains that can be used with the Sanderson's Rapid Bone Stain (RBS). We primarily used the acidified acid fuchsin, however, we had clients that only used a van Gieson counterstain. Light green can also be used. We normally stained for 1 minute ground sections in the RBS, rinsed in water and then counterstained. As Gayle mentioned, intensity of the RBS or the counterstain can be increased with addtional time. Cathy Mayton Wasatch Histo Consultants, Inc. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet