About to use the antibody anti-connexin 43. On the data sheet it
tells me to block using 5% milk, so it's casein blocking. In 1992 Dave Tacha
published a paper (J. Histotech. 15. 132-137. Casein reduces non-specific
background staining in immunolabelling techniques), regarding casein
blocking where he recommended using it at 5%. Later however, Mary Vaughan on
Histonet suggested reducing the concentration, after a series of
experiments, to 0.03% for 30 minutes.
What's the current view on casein blocking, concentration, time
etc.
Ian.
Dr. Ian Montgomery,
Histotechnology,
I.B.L.S. Support Unit,
Thomson Building,
University of Glasgow,
Glasgow,
G12 8QQ.
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