Hi Histonet,
We have a working IHC protocol for F4/80 on mouse tissues. However, when we try to switch this procedure to IF it doesn't work. Would anyone please have any suggestions as to why this won't work? The pretreatment that we use is trypsin at 37 degrees for 15 min. Does this enzyme inhibit the Cy5 which is the secondary fluorophore that we're using? Any advice/help is greatly appreciated! Thanks! Anne _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
