Hi, I am trying very hard to get a clear MMA embedded block. I am appreciate if there is any advice on doing it. I am using 100% MMA (liquid) and 0.5% Perkadox 16 (powder) to do the embedding. I have tried to embed the sample in different conditions. However, it seems to be extremely hard to get a clear embedded block.
Below are the methods that I have tried. 1. Before embedding, bring the MMA and perkadox 16 to room temperature. After a few mix it by invertion, I aliquot the solution to the glass vials that contain samples or simply glass vials that without sample. I put the glass vials (both with and without sample) in 4 degree, room temperature and even 37degree oven. 2. Mix the MMA and perkadox when it is cold, invert it a few times. Aliquot it to glass vials that contain sample or without sample. Put in 4degree, room temperature and 37degree oven. I have also tried to speed up the polymerization process by using vacumm for a few samples... Throughout all the sample that I have, I only manage to get a clear empty glass vial which I put in the 4degree for one month and a clear embedded sample which I put in 4 degree overnight and bring it out to room temperature. The rest of the glass vials (with or without sample) are cloudy. I have repeated the methods that I used to get a clear embedded block, I did not get any clear block (for both with and without sample). I am glad if there is any expert advice or guidance. We need a clear embedded sample. Thank you very much! Regards, Ooi _________________________________________________ Confidential information. Unauthorized use or disclosure prohibited. Refer http://www.singhealth.com.sg/ContactUs/#Disclaimer _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet