Hi, i dont want to use PFA for the brain fixation (rat). Now I tried to perfuse the rat with saline, followed with 70% alcohol. Then I do post-fixation at room temperature for 24 hours. I also tried saline perfusion, then I directly put the whole brain into 70% alcohol. Is this fine?
Also, for dehydration before cutting frozen sections on a cryostat/microtome, should I use 95% alcohol? I am now using 30% sucrose~ 2009-05-15 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
