No you need to thaw/fix in formalin or alcoholic fixative in order to process and embed in paraffin. Patsy
Patsy Ruegg, HT(ASCP)QIHC IHCtech, LLC Fitzsimmons BioScience Park 12635 Montview Blvd. Suite 215 Aurora, CO 80010 P-720-859-4060 F-720-859-4110 email pru...@ihctech.net website www.ihctech.net IHC Resource Group www.ihcrg.org -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Monfils, Paul Sent: Friday, May 15, 2009 10:41 AM To: histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Embeeding frozen tissue in paraffin I assume you mean the tissue was frozen without fixation, not that you want to put it into paraffin without fixation? No tissue will withstand paraffin processing without fixation. The best way to turn a frozen tissue into a paraffin embedded tissue is to drop the frozen tissue (either plain or in embedding medium, whichever is the case) into formalin while still frozen. Let it thaw and fix in the formalin, using a typical fixation time for a tissue that size, then just process and embed it as you would any tissue. > ---------- > From: histonet-boun...@lists.utsouthwestern.edu on behalf of Tomasz Bonda > Sent: Friday, May 15, 2009 6:19 AM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Embeeding frozen tissue in paraffin > > Hello, > is there any good method for embeeding fresh frozen tissue (without any > fixation) in paraffin? > I would be grateful for ANY suggestions. > > T. Bonda > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet