Hi, but I dont want to destory everything.... I am trying to see if I can prevent the binding of another 2nd antibody to the primary antibody for the first antigen! There fore you can perform double staining from same sepecies-derived primary antibodies!
2009-05-17 TF 发件人: Rene J Buesa 发送时间: 2009-05-17 00:54:26 收件人: Histonet@lists.utsouthwestern.edu; tifei 抄送: 主题: Re: [Histonet] Chemicals that inactivate the primary antibody Try bleach and you will destroy everything! René J. --- On Sat, 5/16/09, TF <ti...@foxmail.com> wrote: From: TF <ti...@foxmail.com> Subject: [Histonet] Chemicals that inactivate the primary antibody To: "Histonet@lists.utsouthwestern.edu" <Histonet@lists.utsouthwestern.edu> Date: Saturday, May 16, 2009, 12:48 PM Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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