But the heat also damage the fluorescnece... u need specific amplication kit anyway.
2009-05-17 TF 发件人: Gudrun Lang 发送时间: 2009-05-17 02:00:18 收件人: ti...@foxmail.com 抄送: histonet@lists.utsouthwestern.edu 主题: AW: [Histonet] Chemicals that inactivate the primary antibody For doublestaining the primary undergoes denaturation through a second HIER-step. The second secondary ab doesn't bind to the first primary. Therefore the binding sites must have been destroyed by heat in retrieval-buffer. Gudrun -----Urspr黱gliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von TF Gesendet: Samstag, 16. Mai 2009 18:48 An: Histonet@lists.utsouthwestern.edu Betreff: [Histonet] Chemicals that inactivate the primary antibody Hi all, just wonder what kind of treatments/chemicals can complete block the binding of 2nd antibody to binded primary antibody on antigen? I tried HCl , but does not damage all the primaryantibody binding sites - i can still see staining pattern finally. 2009-05-17 TF _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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