hello everyone,
I wanted to know the exact stepwise protocol for antigen
retrieval using a pressure cooker. i tried using some protocol but there is no
staining. Kindly tell me the detailed protocol, like when i should place the
slides into the pressure cooker and from when i should count the time.
And i am using mouse monoclonals CD44 & CD24 labvision in canine tissue, has
anyone got experience with these antibodies as citrate buffer doesnt at all
seems to work.
plz send me some information on this.
Regards,
Dr. Anjan Kumar.K.R
M.V.Sc Scholar
Dept. of Veterinary Pathology
Madras Veterinary College
Chennai-7
India
email: drvet_an...@hotmail.com
Phone: +91-9940475801
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