I read somewhere that you are not to pH 0.175 M sodium acetate solution when using it to prepare a nickel-DAB chromogen solution. The problem is that whenever I make up 0.175 M sodium acetate (14.35 g of sodium acetate in 1 L of dH2O), the pH of my solution is generally around 8.0 to 8.1. From what I have read, the solution's pH should be closer to 7.0 (presumably w/o adjusting the pH of this solution)? Does anyone know what the correct pH of 0.175 M sodium acetate should be when preparing a nickel DAB chromogen solution? Am I missing something here?
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