Hi Histonet... Quick question!! I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in 95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for 5') my protocol calls for me to dehydrate the tissue in ethanol (95%x2, 100%x2) but this pulls out basically all of my CV stain! Does anyone have a solution for this?
Also, I have horrible frozen artifacts... is there anything I can do even though the tissue has already been sectioned? What about during the sectioning process? Thanks in advance! Nicole J. Patten Post-Baccalaureate Fellow/IRTA NIAAA/National Institutes of Health _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
