Dear Jerry and histonetters, I don't believe this is unethical or illegal. It is written into our IACUC approved protocols as follows:
Chemical Method of Euthanasia: "Perfusion under general anesthesia, Avertin or Halothane induced. Bilateral thoracotomy." Could it only be forbidden by your facility? I wonder if other facilities have rules against such things. But we insist that our investigators use perfusion under anesthsia because the deeper tissues are much better fixed and blood removal is more thorough and complete by this method. Jo Dee ~~Jo Dee Fish~~ Senior Research Technologist The J. David Gladstone Institutes Co-manager Histology and Microscopy Core Telephone: (415) 734-2567 Fax: (415) 355-0824 E-mail: jf...@gladstone.ucsf.edu Mailing address: The J. David Gladstone Institutes 1650 Owens Street San Francisco, CA 94158 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of JR R Sent: Tuesday, June 30, 2009 10:49 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Good Perfusion... No, I wouldn't nick the atrium. Instead, cut the femoral artery at the groin. And of course, you cannot perfuse a living, anesthetized animal. That would be unethical and also illegal. Jerry Ricks Research Scientist University of Washington Department of Pathology _________________________________________________________________ Insert movie times and more without leaving HotmailR. http://windowslive.com/Tutorial/Hotmail/QuickAdd?ocid=TXT_TAGLM_WL_HM_Tutori al_QuickAdd_062009_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet