I have no scientific experience with this, but in my opinion it has to make a difference, if the proteins are crosslinked with single methylen-bridges or with "longer" bridges, that result of "more-C-compounds".
Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Yak-Nam Wang Gesendet: Mittwoch, 15. Juli 2009 01:32 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] formalin substitutes - tissue structure Dear Histonetters, I have a question about alternatives to formalin fixation and fine changes in tissue structure. We have been obtaining formalin fixed human skin and fat samples from several companies. We use stereological methods to make tissue measurements such as dermal thickness and adipose cell size from sections stained with a variety of basic stains. However, there is now another company that we would like to do obtain more tissue from but they can only provide tissue fixed with a formalin alternative such as FineFix or Prefer. Measurement data collected from formalin and formalin alternative fixed tissue would be used together if we obtained tissue from this other company. >>From the Histonet archives I see that sometimes the use of formalin alternatives can affect immuno staining, but does anyone know how it would affect fine structure of tissue. My thoughts were that there may be a slight difference in 'shrinkage' that occurs given the main ingredient is ethanol on some of the alternatives, so fat fixed in one of these alternatives would give an erroneously smaller adipose cell size. Any insight would be greatly appreciated. Thank you Yak-Nam Wang University of Washington _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet