The addition of dextrin dates from Catalano & Lillie 1975 Stain Technol. 50(5): 297-299. http://pdfserve.informaworld.com.proxy1.lib.uwo.ca:2048/882566_770885140_788730753.pdf They showed that adding dextrin more than trebled the dye content of a supersaturated solution of oil red O in 60% isopropanol, and also increased the shelf life of the solution. These authors did not say what kind of dextrin they used. Dextrin had earlier been added to Weigert-type elastin stains by French 1928 Stain Technol. 4(1): 11-12, who also did not specify a type of dextrin. Lamar Jones, in Chapter 11 of Theory and Practice of Histological Techniques (5th ed, ed. Bancroft & Gamble 2002), p.208 states: "Dextrin, bacteriological grade or Type III from Sigma, from corn starch." Does anyone know of a published comparison of different types of dextrin? John Kiernan Dept of Anatomy & Cell Biology University of Western Ontario London, Canada = = = ----- Original Message ----- From: abi jag <abija...@yahoo.co.in> Date: Friday, July 24, 2009 7:45 Subject: [Histonet] Oil red o_Dextrin_reg To: histonet@lists.utsouthwestern.edu
> Dear Members, > I am trying to standardize the oil red o staining method in > murine frozen tissues using Churukian method. > > I am having following queries. > > 1. The type and source of aqueous dextrin used in the staining > procedure.Search in sigma catalogue provided different types of > dextrin (dextrin from corn,maize and pottao starch etc). I want > to know which should be used exactly in this procedure. > Unfortunately, I am not having the article published in Journal > of histotechnology to refer. Can any one please throw some light > on the type of the dextrin to be used and the source to buy if any. > > 2. Again the procedure mentions a counter stain called acidified > Lillie-Mayer hematoxylin. If there is any composition available > to prepare the same or that could be purchased directly from the > manufacturers (I have seen Dako is having the one). > > Please clarify these two things > > Thanks for all your help > > Abijag > > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
