When this happens in our lab, we put Drierite (anhydrous calcium sulfate) into the clearing agents. Just put enough to have not quite a solid layer on the bottom of the staining well, so it will not block the staining rack from going all the way into the clearing agent. We get ours from W.A. Hammond Drierite Company LTD, Xenia, Ohio 45385. Telephone number 937-376-2927. I hope this helps!
Josie Britton HT Cheshire Medical Center Keene, NH 03431 -----Original Message----- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hayes, Tina J. Sent: Monday, August 24, 2009 12:41 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] water on slides? We are having a problem with what seems to be water on our slides. We have had very high humidity levels in the air. It seems that we look at the slides before taking them to the pathology office, one pathologist reviews them and sees little or no water droplets, but as they sit and another pathologist reads them later, like the following day, the droplets are very apparent. We use Clearite-3. We have no xylene in our lab. We also coverslip with Permount. Is it possible that the clearite-3 and/or Permount is absorbing moisture from the atmosphere to the slides? And if so, do you have any suggestions for combating this issue? _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet CONFIDENTIALITY NOTICE: This electronic message, including any attachments, is for the sole use of the intended recipients and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by electronic mail and destroy all copies of the original message. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet