I'm getting the same eosin bleeding and water on the slides. I'm using Hemo-D and CitriSolv. I have changed the reagents and am still getting the eosin bleeding, the water on the slide is a new phenomenon.
Any suggestions? Paula Sicurello VA Medical Center San Diego Veterans Medical Research Foundation (VMRF) Core for Micro Imaging(C-MI) 3350 La Jolla Village Dr., MC151 San Diego, CA 92161 858-552-8585 x2397 C-MI for your imaging needs. --- On Mon, 8/24/09, Angela Bitting <akbitt...@geisinger.edu> wrote: > From: Angela Bitting <akbitt...@geisinger.edu> > Subject: Re: [Histonet] water on slides? > To: histonet@lists.utsouthwestern.edu, "Tina J. Hayes" <tina.ha...@va.gov> > Date: Monday, August 24, 2009, 6:00 PM > We are having a very similar problem > too. We change our alcohols and Histoclear like mad, but are > having eosin bleeding out of the sections. I'm starting to > wonder if it is the mounting media on our glass > coverslipper. I ordered new bottle gaskets, so I guess I'll > see what happens then. > > Angela Bitting, HT(ASCP) > Technical Specialist, Histology > Geisinger Medical Center > 100 N Academy Ave. MC 23-00 > Danville, PA 17822 > phone 570-214-9634 > fax 570-271-5916 > > No trees were hurt in the sending of this email > However many electrons were severly inconvienienced! > > > >>> "Hayes, Tina J." <tina.ha...@va.gov> > 8/24/2009 12:41 PM >>> > We are having a problem with what seems to be water on our > slides. We > have had very high humidity levels in the air. It > seems that we look at > the slides before taking them to the pathology office, one > pathologist > reviews them and sees little or no water droplets, but as > they sit and > another pathologist reads them later, like the following > day, the > droplets are very apparent. > > > > We use Clearite-3. We have no xylene in our > lab. We also coverslip > with Permount. > > > > Is it possible that the clearite-3 and/or Permount is > absorbing moisture > from the atmosphere to the slides? > > And if so, do you have any suggestions for combating this > issue? > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > IMPORTANT WARNING: The information in this message (and the > documents attached to it, if any) is confidential and may be > legally privileged. It is intended solely for the addressee. > Access to this message by anyone else is unauthorized. If > you are not the intended recipient, any disclosure, copying, > distribution or any action taken, or omitted to be taken, in > reliance on it is prohibited and may be unlawful. If you > have received this message in error, please delete all > electronic copies of this message (and the documents > attached to it, if any), destroy any hard copies you may > have created and notify me immediately by replying to this > email. Thank you. > -----Inline Attachment Follows----- > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet