Hi Aazath, This is our HE protocol, it's for 16 stations. And I'm interested in your PAP-protocol. Would you be so nice and send it to me?
25 min oven 2 x 5 min Xylol-substitute 1 x 1 min 96% 1 x 1 min 80% 1 x 1 min 50% 1 x 1 min dest. water 1 x 10 min Mayers Hemalaun (exact) 1 x 5 min tapwater running 1 x 30 sek 2% Eosin in water (exact) 1 x 30 sek tapwater running (exact) 1 x 30 sek 96% (exact) 3 x 1 min 100% 2 x Xylol-substitute Coverslipper The times without "exact" are minimum, "dip" is on with all stations. Alcohols and Xylol-subst. are changed every day. Hope this helps Gudrun Lang -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Aazath Raj Gesendet: Samstag, 29. August 2009 19:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] (no subject) Dear All, we have got a new leica multistainer ST 5020.we are using it for both H&E and as well as PAP.Out of 34 station i have only 15 station for H&E.Is anybody having a Staining protocol for H&E which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo Hospitals Chennai India _________________________________________________________________ Log on to MSN India for a lowdown on what’s hot in the world today http://in.msn.com_______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet