Hello. Histonetters. I have recently used zinc fixed tibia and calvaria for the staining of CD31. My CD31 does seem to have worked, however there seems to be marrow loss and digestion of the marrow also.
I zinc fixed the bones for 48 hours and then used EDTA pH 7 for decalcification for 2 weeks. I am unsure whether I have fixed the bones for too long, whether the decal has been affected by the zinc fix (as the bones were "jelly-like" before processing) or whether the decalcification needs shortening when using zinc fixation. If anyone has any experience in using zinc fixation on tibia for Immuno staining and can offer some advice that would be much appreciated. Thanks Julia _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet