I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5
(far-red) fluorophores, but they cannot make them in the red (Alexa Fluor
555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted
microscope we have LEDs and we also have a mercury lamp to cover the red
range. The Zeiss switches nicely and very smoothly between the light
sources during viewing and image acquisition.
Sorry for the highly untechnical description, but that is all I know about
it. :-)
Regards,
Merced
--On Wednesday, September 09, 2009 12:21 PM -0400 Merced M Leiker
<[email protected]> wrote:
LEDs don't quench your fluorescence as quickly as mercury.
--On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz
<[email protected]> wrote:
Dear Colleagues,
We're attempted to buy a new inverted fluorescence microscope for our
research lab. Distrubitor of leica offered us a new type system working
with led lights which is about same price. Does anybody know advantages
and disadvantages of this system compared to conventional (with mercury
lamp) fluorescence microscopes.
Thanks in advance.
Necat Yilmaz
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
[email protected]
716-829-6118 (Ph)
716-829-2665 (Fx)
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However, many electrons were severely inconvenienced.
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
[email protected]
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
_______________________________________________
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