Interesting, Tim! (I could be wrong, but I was pretty sure the said that
when I questioned him about it!) I just checked the Zeiss website and
apparently they carry 10 diodes to emit light at a particular frequency,
including the reds. Now I need to ask him again why we don't have the red
LED! Links below:
<http://zeiss-campus.magnet.fsu.edu/tutorials/colibri/index.html>
<http://www.zeiss.de/colibri>
--On Wednesday, September 09, 2009 11:25 AM -0700 "Morken, Tim"
<[email protected]> wrote:
Merced wrote: " I forgot to mention. An LED can be made to excite FITC,
DAPI, and Cy5 (far-red) fluorophores, but they cannot make them in the
red (Alexa Fluor 555, Cy3, TRITC, etc.) This is per our Zeiss rep."
I'm not sure why the Zeiss rep said that. Maybe Zeiss itself does not
carry the LED's in that range but I have used up to 655nm LED.
See this for details on other LED's for fluorescence work:
http://www.fraensrl.com/images/fraen_fluorochromes.pdf
Tim Morken
UCSF Medical Center
San Francisco, CA
-----Original Message-----
From: [email protected]
[mailto:[email protected]] On Behalf Of Merced M
Leiker Sent: Wednesday, September 09, 2009 10:44 AM
To: Nejat Yilmaz; [email protected]
Subject: Re: [Histonet] mercury vs led lights
I forgot to mention. An LED can be made to excite FITC, DAPI, and Cy5
(far-red) fluorophores, but they cannot make them in the red (Alexa Fluor
555, Cy3, TRITC, etc.) This is per our Zeiss rep. So on our inverted
microscope we have LEDs and we also have a mercury lamp to cover the red
range. The Zeiss switches nicely and very smoothly between the light
sources during viewing and image acquisition.
Sorry for the highly untechnical description, but that is all I know
about it. :-)
Regards,
Merced
--On Wednesday, September 09, 2009 12:21 PM -0400 Merced M Leiker
<[email protected]> wrote:
LEDs don't quench your fluorescence as quickly as mercury.
--On Wednesday, September 09, 2009 4:58 PM +0300 Nejat Yilmaz
<[email protected]> wrote:
Dear Colleagues,
We're attempted to buy a new inverted fluorescence microscope for our
research lab. Distrubitor of leica offered us a new type system working
with led lights which is about same price. Does anybody know advantages
and disadvantages of this system compared to conventional (with mercury
lamp) fluorescence microscopes.
Thanks in advance.
Necat Yilmaz
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
[email protected]
716-829-6118 (Ph)
716-829-2665 (Fx)
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
[email protected]
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
_______________________________________________
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[email protected]
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
[email protected]
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
_______________________________________________
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