Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue
processor and have been test processing tissues under different hour
protocols. We encountered problems in the 8-hr and 5-hr protocols.Both produced
tissues that were too difficult to section in the microtome. My histotechs
showed me tissues that were like brittle plastics. Despite reprocessing, the
tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues
for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were
too thickly sampled and poorly fixed. May ask for feedback/advice on the ff;?
1. Possible reasons?
2. Steps to prevent this in the future?
3. Reprocessing protocol in Peloris.
Thank you so much.
Dr. Evette Demaisip
Pathologist,Philippines
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