Your problems probably are caused by the dehydration steps. Check them René J.
--- On Wed, 9/9/09, evette demaisip <e_dema2...@yahoo.com> wrote: From: evette demaisip <e_dema2...@yahoo.com> Subject: [Histonet] Peloris tissue processor To: histonet@lists.utsouthwestern.edu Date: Wednesday, September 9, 2009, 8:51 PM Hi. We're currently setting up a histopath lab. We've purchased Peloris tissue processor and have been test processing tissues under different hour protocols. We encountered problems in the 8-hr and 5-hr protocols.Both produced tissues that were too difficult to section in the microtome. My histotechs showed me tissues that were like brittle plastics. Despite reprocessing, the tissues came out the same in the 8-hr protocol. We reprocessed the 5-hr tissues for 12 hrs, the tissues looked microscopically ok. I suspect the tissues were too thickly sampled and poorly fixed. May ask for feedback/advice on the ff;? 1. Possible reasons? 2. Steps to prevent this in the future? 3. Reprocessing protocol in Peloris. Thank you so much. Dr. Evette Demaisip Pathologist,Philippines Get your new Email address! Grab the Email name you've always wanted before someone else does! http://mail.promotions.yahoo.com/newdomains/aa/ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
