I use it straight out of the bottle. My supervisor uses it straight. The institution where I first learned to coverslip used it straight as well. I'm pretty sure the UB Histology Lab here uses it straight as well...

Merced

--On Thursday, September 10, 2009 1:03 PM -0700 Leslie Chen <lc...@mednet.ucla.edu> wrote:

Hello,
Did anyone ever use Permount straight without cutting it with some
solvent? Journal articles mention using Permount to coverslip, and the
post-doc I work with thinks that it isn't diluted.

I think maybe people just universally "know" that it should be diluted so
it isn't mentioned in the Materials and Methods.  Any thoughts?

Leslie
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214  USA
lei...@buffalo.edu
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