I do recall having to do that in the past; if the Permount bottle had been
left open in the hood and became too viscous we would add just enough
xylene to get it flowing again. Didn't happen often, if you're careful to
keep the bottle closed when not in use.
Regards,
Merced
--On Monday, September 14, 2009 9:15 AM +1000 Tony Henwood
<antho...@chw.edu.au> wrote:
It is possible that the mountant was too thick and so xylene was added
to thin it?
It would save the lab money in mountant costs but there is a possibility
that with less mountant on the slide you may have shrinkage of the
mountant causing eventual cracking of the mountant and possible
detachment of the coverslip.
I would review a sample of slides one and two years old and record the
quality (another audit!)
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-----Original Message-----
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Merced M
Leiker
Sent: Saturday, 12 September 2009 12:30 AM
To: Leslie Chen; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Re: Permount question
Just so a stereotype doesn't get started here...I was 1st taught in the
(straight) use of Permount by such an Asian technician (who couldn't
speak
English, but was such a wonderful teacher I learned more from watching
her
than anything!) :-)
--On Thursday, September 10, 2009 1:52 PM -0700 Leslie Chen
<lc...@mednet.ucla.edu> wrote:
Thanks for all the replies, I guess these weird Asian technicians who
are highly looked up to taught me something wrong and it's been
propagated in the department. Thanks again!
On Thu, Sep 10, 2009 at 1:03 PM, Leslie Chen <lc...@mednet.ucla.edu>
wrote:
Hello,
Did anyone ever use Permount straight without cutting it with some
solvent? Journal articles mention using Permount to coverslip, and
the post-doc I work with thinks that it isn't diluted.
I think maybe people just universally "know" that it should be
diluted so it isn't mentioned in the Materials and Methods. Any
thoughts?
Leslie
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
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Merced M Leiker
Research Technician II
Cardiovascular Medicine
348 Biomedical Research Building
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214 USA
lei...@buffalo.edu
716-829-6118 (Ph)
716-829-2665 (Fx)
No trees were harmed in the sending of this email.
However, many electrons were severely inconvenienced.
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