To possibly kill (or at least wound a little?) two birds with the one stone...
We have used BD Transduction anti-eNOS, # 610296 successfully on FFPE rat tissue, if that is any help. It seems to label the endothelium in all vessels in the tissues we have looked at so far, as far as we can tell (eNOS being a constitutive form of NOS) and therefore may be considered a vascular marker. Cheers, Jason Jason Palmer Histology Laboratory Coordinator O'Brien Institute 42 Fitzroy St, Fitzroy Victoria 3065 Australia tel +61 3 9288 4045 fax +61 3 9416 0926 email: jason.pal...@svhm.org.au ?????? [Histonet] in search of cd31 for rat tissue Has anyone used RECA-1 (from Novus) to stain endothelial cells in rat tissue. I also heard about RDI-RTCD31-3A12 from Fitzgerald but it requires Zinc fixative and frozen sections, I would really like to use ffpe rat tissue already archived??? Regards, Patsy ------------------------------ From: "Nejat Yilmaz" <nyil...@mersin.edu.tr> Subject: [Histonet] novocastra eNOS antibody on rat tissues??? Dear All, We're planning to study FFPE rat aorta specimens with anti endothelial nitric oxide synthase (NOS-3) antibody. We want to perform that with Novocastra brand anti-eNOS antibody (Product Code: NCL-NOS-3). According to datasheet the antibody works on human tissues only, other species not tested yet. Does anybody have any experince with this antibody on different species other than human? Thanks in advance. Necat Yilmaz MD PhD University of Mersin School of Medicine Disclaimer : The contents of this e-mail including any attachments are intended only for the person or entity to which this e-mail is addressed and may contain confidential, privileged and/or commercially sensitive material. If you are not, or believe you may not be, the intended recipient, please advise the sender immediately by return e-mail, delete this e-mail and destroy any copies. ______________________________________________________________________ This email has been scanned by the MessageLabs Email Security System. For more information please visit http://www.messagelabs.com/email ______________________________________________________________________ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
