We use Dako Von Willebrand Factor VIII in Rabbit on multiple species and it
works fabulously. Enzyme pretreatment with 0.03% pronase e for 10 mins at
37C / Primary at 1:200 to 1:500 depending on the species being stained.
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax: (517) 432-1368
Email: port...@msu.edu
Web: www.humanpathology.msu.edu
----- Original Message -----
From: "Jackie M O'Connor" <Jackie.O'con...@abbott.com>
To: <histonet@lists.utsouthwestern.edu>;
<histonet-boun...@lists.utsouthwestern.edu>
Sent: Wednesday, October 28, 2009 4:35 PM
Subject: [Histonet] Canine endothelial antibody
A week or so ago I thought I saw a post about an antibody that works for
canine endothelial cells, but I can't find it in the archives - was I just
dreaming, because I need this so bad? I've tried Factor VIII and a CD34
on FFPE, but with poor results. Any help would be most appreciated.
Thanks,
Jackie O'
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