Jackie,
Dako's Factor VIII (von Willebrand Factor), cat. #A0082 works for me on
canine tissue (and cat, pig, cow, horse, sheep, chicken, mouse, etc.). I
use Proteinase K enzyme digestion pretreatment for 5' at room temperature;
primary rabbit Ab incubation 30', EnVision+/HRP anti-Rabbit 30' (with 4%
normal dog serum added per volume for blocking), AEC 10'.
Hope this helps,
Jan Shivers
Senior Scientist
Pathology Teaching Program
Histology/IHC/EM Section Head
University of Minnesota
Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul, MN 55108
612-624-7297
shive...@umn.edu
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----- Original Message -----
From: "Jackie M O'Connor" <Jackie.O'con...@abbott.com>
To: <histonet@lists.utsouthwestern.edu>;
<histonet-boun...@lists.utsouthwestern.edu>
Sent: Wednesday, October 28, 2009 3:35 PM
Subject: [Histonet] Canine endothelial antibody
A week or so ago I thought I saw a post about an antibody that works for
canine endothelial cells, but I can't find it in the archives - was I just
dreaming, because I need this so bad? I've tried Factor VIII and a CD34
on FFPE, but with poor results. Any help would be most appreciated.
Thanks,
Jackie O'
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