Hi all, I am using an monoclonal antibody to test a membrane protein (tested
appilication include Flow cyt and western blot).
It was succsessfuly used in Immunocytochemistry (cell culture) before. However
when I applied it in Immunohistochemistry (Frozen sections, human tissue),
there was no signal at all (The fixation is the same).
Is there so big difference between ICC and IHC-Fr? Is it my problem or the
antibody's?
Many thanks!
Celia
Chinese University of Hong Kong
Hong Kong
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