Kris, why don't you leave the skin biopsies in NBF over night? At our institute it is usual practice to let this specimens fix until next day, if they arive after 1 p.m. I cannot see a negative effect. For me 4 hours in formalin are too less for a good fixation. How long is the duration of fixation in the infiltration processor? Aren't the biopsies rather brittle while cutting, due to secondary fixation with ethanol?
Gudrun -----Ursprüngliche Nachricht----- Von: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] Im Auftrag von Kalleberg, Kristopher Gesendet: Mittwoch, 25. November 2009 00:51 An: histonet@lists.utsouthwestern.edu Betreff: [Histonet] Biopsy fixation Hello All, I have a simple fixation question. Typically when we process 3 mm skin biopsies we will fix in 10% NBF for 4 hours and then wash in PBS and then process over night into paraffin and then embed. If we are unable to immediately process the 3mm skin biopsies after 4 hr fixation what is the maximum time we can leave the biopsies in 70% alcohol before processing into paraffin? Is there a better reagent, such as PBS, other than alcohol to leave the biopsy in for a few days before tissue processing. Thank you in advance. Kris _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
